Plasmid_Backbone
pSB8K15FRT
Part:BBa_K864022:Design
Designed by: Erik Lundin Group: iGEM12_Uppsala_University (2012-09-24)
Low copy BioBrick temperature sensitive standard vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711
Illegal NheI site found at 2425
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3717 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3711
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3711
Plasmid lacks a suffix.
Illegal XbaI site found at 3726
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2319 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.
The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
Source
Based on BBa_K864017